Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications tissue engineering. However, decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study assess structural integrity, biocompatibility, and potential use acellular engineering applications. Hence, bovine pericardium (BP), porcine (PP), tunica vaginalis (PTV) were decellularized using Trypsin, Triton X (TX), sodium dodecyl sulfate (SDS) (Trypsin + TX SDS) protocol. results reveal effective elimination cellular antigens with preservation ECM integrity confirmed via staining electron microscopy. elasticity PP (DPP) was markedly (p < 0.0001) increased. tensile strength DBP, DPP not affected after decellularization. All tissues biocompatible persistent growth adipose stem cells over 30 days. cell adherence either peripheries materials or within their matrices. Moreover, vivo investigation biocompatibility degradability scaffolds. Conclusively, Trypsin SDS successful new protocol pericardia scaffolds higher cardiovascular DBP DPTV reconstruction higher-stress-bearing abdominal walls.